The axon projection mode only partially correlates with the apical dendrite feature. On the other hand, each projection mode may consist of four dendritic types defined in this study. Correlating the two types of classifications, we have found that there are at least two projection modes comprised in the former defined neuron types except for L5-tt. By examining the axonal projection patterns of individual neurons, they can be categorized into three modes: ipsilateral circuit connection neurons, callosal projection neurons and corticofugal projection neurons. Based on the complete topology of neurons and the inherent positioning function of the imaging method, we classified the observed neurons into six types according to their apical dendrites and somata location: star pyramidal cells in layer 5 (L5-sp), slender-tufted pyramidal cells in layer 5 (L5-st), tufted pyramidal cells in layer 5 (L5-tt), spiny stellate-like cells in layer 6 (L6-ss), star pyramidal cells in layer 6 (L6-sp), and slender-tufted pyramidal cells in layer 6 (L6-st). A total of 103 neurons were reconstructed in layers 5 and 6 of the visual cortex with single-axon-level resolution. In this paper, we applied the brain-wide positioning system (BPS) to image the entire brain of two Thy1-eYFP H-line male mice at 0.2 μm × 0.2 μm × 1 μm voxel resolution. However, the diverse neuron types and their morphological properties have not been fully deciphered. The excitatory neurons in the visual cortex are of great significance for us in understanding brain functions. 2MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.Yalun Zhang 1,2 Siqi Jiang 1,2 Zhengchao Xu 1,2 Hui Gong 1,2 Anan Li 1,2 Qingming Luo 1,2 Miao Ren 1,2 Xiangning Li 1,2 Hao Wu 1,2 Jing Yuan 1,2 Shangbin Chen 1,2*

Axon to dendrite ratio circuits